G.C. Clarus 500 - Perkin Elmer

The gas chromatograph used is the Perkin Elmer Clarus 500 with autosampler and flame ionization detector FID (Flame Ionisation Detector).The analysis are performed using capillary columns where the stationary phase is deposited in the form of a thin film (0.1-5 microns) on the inner wall of a long capillary of 15 to 100 m. The carrier gas, helium, is conveyed into a nozzle and mixed with air and hydrogen, which feed a flame that produces an ionic current which is converted into voltage, amplified and processed. When an analyte reaches the flame, it is burned and ionized by varying the intensity of the current and allowing the detection of the signal which is subsequently processed by the software Total Chrom. The concentration of the samples is established on the basis of the internal standard method, by means of standard components and specially using appropriate calibration curves return the values of concentration of various analytes.